设为首页加入收藏联系站长 English VersionMybiosoftware
首页常见问题与解答 友情链接 站内检索关于本站 

 

 

生物软件网>>>>生物专业电子书>>>>Alkami Quick Guide for PCR PCR实验的实验室参考手册

Alkami Quick Guide for PCR PCR实验的实验室参考手册
   

资料大小: 300K
资料语言: 英文
资料类别: 生物专业电子书
运行环境: Win9x/Me/NT/2000/XP/2003
加入时间: 2006-11-7
原始网站: homepage

详细介绍

 

PCR实验的实验室参考手册,PDF格式,可供实验室参考。

PREFACE.................................................................................... 4
LAB SUPPLY SELECTION ........................................................ 5
LABORATORY GLOVES ................................................................ 5
PCR MICROTUBES ..................................................................... 9
PIPETTS & TIPS ........................................................................ 12
PCR SEALING & STORAGE PRODUCTS...................................... 16
PRIMERS .................................................................................. 19
PRIMER DESIGN TIPS ................................................................ 19
REVIEWS OF ON-LINE AND FREEWARE PRIMER DESIGN TOOLS...... 22
PCR METHODS ........................................................................ 32
STANDARD PCR ....................................................................... 32
LA PCR................................................................................... 33
HOT START PCR ...................................................................... 34
TOUCHDOWN PCR.................................................................... 39
RT PCR................................................................................... 40
IN SITU PCR............................................................................. 45
QUANTITATIVE PCR.................................................................. 49
PCR VARIABLES ..................................................................... 51
Primers................................................................................. 52
Polymerases ........................................................................ 52
Templates ............................................................................ 53
Magnesium ions................................................................... 54
Preincubation Temperatures and Times.............................. 55
Melting Temperatures and Times........................................ 55
Temperature - Annealing/Hybridization ............................... 56
Temperature - Extension/Polymerization............................. 56
Estimating extension times for LA PCR............................... 57
Cycles .................................................................................. 57
Reaction Volumes................................................................ 58
PCR POLYMERASES............................................................... 60
TAQ (THERMUS AQUATICUS)...................................................... 62
KLENTAQ (THERMUS AQUATICUS, N-TERMINAL DELETION MUTANT)
..63
STOFFEL FRAGMENT ................................................................. 64
TTH (THERMUS THERMOPHILUS).................................................64
PFU (PYROCOCCUS FURIOSUS) ..................................................65
VENT (THERMOCOCCUS LITORALIS)............................................66
DEEP VENT (PYROCOCCUS SPECIES GB-D) ...............................67
ULTMA (THERMOTOGA MARITIMA)..............................................68
PCR TROUBLESHOOTING......................................................69
Non-Specific Product Yields.................................................69
Little or No Product Yield......................................................70
Multiple Product Yields or High Molecular-Weight Smear is
Observed..............................................................................72
Primer-Dimers ......................................................................73
About Inhibitors.....................................................................74
About Enhancers..................................................................74
How to Reduce Contamination.............................................76
Techniques Used to Prevent Contamination........................77
PCR INHIBITORS & ENHANCERS ..........................................79
GENERAL/INTRO, FECES, FORMALIN-FIXED PARAFFIN SAMPLE ...79
FECES, NORWALK VIRUS, ROTAVIRUS, URINE ............................82
RESPIRATORY FLUID, BLOOD, SHELLFISH...................................86
BODY FLUIDS AND FOOD............................................................90
PLANTS & SOIL .........................................................................94
SPECIAL PCR TOPICS.............................................................97
WHY AREN'T PCR-BASED DIAGNOSTICS MAIN STREAM?............97
PCR-BASED DETECTION KITS PART I.......................................100
PCR-BASED DETECTION KITS PART II.....................................103
FISH, PRINS & CYTOGENICS: DO YOU HAVE A LIGHT? ..........106
CLINICAL APPLICATIONS OF DOT BLOTS ...................................110
APPENDIX A ¨C UNITS AND FORMULAS..............................113
APPENDIX B ¨C THE GENETIC CODE....................................116
APPENDIX C ¨C ONLINE GENOMIC DATABASES................117
APPENDIX D ¨C PCR PRODUCT SUPPLIERS .......................124
APPENDIX E ¨C ONLINE BIOSUPPLIERS..............................137
APPENDIX F - CONTRIBUTORS ...........................................139
INDEX

 

 

下载地址

点击这里下载

(如果无法下载,请到原始网站下载或者购买光盘)

 
生物光盘
以上资料,在生物图书DVD光盘1 购买此光盘请 点击

 

 

PAUP * 软件代理购买

本站生物光盘出售
原版书代购
网站广告联系
三维分子
DNA分析
RNA分析
蛋白质分析
生物教学
生物化工
PCR相关
序列格式转换
进化分析
质粒绘图
图像处理
序列比对/综合分析
检索与阅读
生物芯片
杂七杂八
数据处理
化学绘图
化学应用
趣味生物软件
生物图片与动画
生物音像资料
生物考研资料
资料库
专业电子书
讲义与课件
SEWER中文版
SMS中文版
生物学数据库
在线综合工具
在线蛋白工具
在线生物资源

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 


★Copyright (C) 1999-2016 生物软件网
★保留所有权利,未经允许,不得引用本站文字★

站长信箱:biosoftcn@gmail.com(谈杰)